"MTS training courses are an effective method for cross-training our laboratory staff and teaching new staff and students. Even our nurse practitioners use them for training!"

 DeAnne Maxwell
 St. Francis Hospital
 Beech Grove, IN




 


Protein Electrophoresis

Training Course Specifics
Summary: Interpretation of protein electrophoresis patterns from serum, urine, and cerebrospinal fluid

Intended Audience: MTs, MLTs, MDs and others reading SPE/IFE patterns

Length of Course: 3 hours

Number of Exam Questions: 10

Authors: Michael L. Astion, MD, PhD
Joseph P. Rank, MD
Mark H. Wener, MD
Lawrence M. Killingsworth, PhD

Continuing Education Credit
Number of credit hours: 3 hours
Accredited by: P.A.C.E. (State of California)
  State of Florida

Learning Objectives

Describe the methods of agarose gel electrophoresis, immunofixation, and densitometry.

Name the clinical conditions most commonly associated with monoclonal gammopathy, and some key clinical features of each condition.

View an agarose gel, an immunofixation, and a densitometry and determine if a monoclonal gammopathy is present. If it is present, the user will be able to identify the immunoglobulin type.

View an agarose gel of serum, urine, or cerebrospinal fluid and identify the electrophoresis pattern (e.g., inflammation, liver disease, hemolysis, alpha-1 antitrypsin deficiency, tubular proteinuria, CSF oligoclonal banding, etc).


Course Outline 
Introduction
Program Overview
Agarose Gel Electrophoresis
Immunofixation
Densitometry
Serum and Urine Patterns
Normal Pattern
Monoclonal Gammopathy
Inflammation
Protein Losing Disorders
Liver Disease
Other Serum Patterns
Other Urine Patterns
Cerebrospinal Fluid
Normal CSF
Abnormal CSF
Review of Disease States
Monoclonal Gammopathy
Inflammation
Liver Disease
CSF Oligoclonal Banding
Final Exam
Exam 1