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"MTS training courses are an effective method for cross-training our laboratory staff and teaching new staff and students. Even our nurse practitioners use them for training!"
DeAnne Maxwell St. Francis Hospital Beech Grove, IN |
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Protein Electrophoresis
Training Course Specifics
| Summary: |
Interpretation of protein electrophoresis patterns from serum, urine, and cerebrospinal fluid |
| Intended Audience: |
MTs, MLTs, MDs and others reading SPE/IFE patterns |
Length of Course: 3 hours
Number of Exam Questions: 10
| Authors: |
Michael L. Astion, MD, PhD Joseph P. Rank, MD Mark H. Wener, MD Lawrence M. Killingsworth, PhD |
3 hours
| Accredited by: |
P.A.C.E. (State of California) |
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State of Florida |

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Describe the methods of agarose gel electrophoresis, immunofixation, and densitometry. |

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Name the clinical conditions most commonly associated with monoclonal gammopathy, and some key clinical features of each condition. |

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View an agarose gel, an immunofixation, and a densitometry and determine if a monoclonal gammopathy is present. If it is present, the user will be able to identify the immunoglobulin type. |

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View an agarose gel of serum, urine, or cerebrospinal fluid and identify the electrophoresis pattern (e.g., inflammation, liver disease, hemolysis, alpha-1 antitrypsin deficiency, tubular proteinuria, CSF oligoclonal banding, etc). |
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Introduction
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Program Overview |
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Agarose Gel Electrophoresis |
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Immunofixation |
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Densitometry |
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Serum and Urine Patterns
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Normal Pattern |
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Monoclonal Gammopathy |
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Inflammation |
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Protein Losing Disorders |
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Liver Disease |
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Other Serum Patterns |
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Other Urine Patterns |
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Cerebrospinal Fluid
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Normal CSF |
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Abnormal CSF |
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Review of Disease States
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Monoclonal Gammopathy |
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Inflammation |
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Liver Disease |
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CSF Oligoclonal Banding |
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Final Exam
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Exam 1 |
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