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Introduction to Transfusion Service

Training Course Specifics
Summary: Pre-transfusion testing. The laboratory’s role in the evaluation of a suspected transfusion reaction. ABO compatibility. Antibody screen and crossmatch.

Intended Audience: All laboratory staff and students

Length of Course: 2 hours

Number of Exam Questions: 10

Authors: Theresa Nester, MD
Adam Orkand, BA

Editors
Michael Astion
Dawn Rumsey
Darolyn Crandall
Jon Anderson
Anne Zimmerman
Joyce Behrens

Continuing Education Credit
Number of credit hours: 2 hours
Accredited by: P.A.C.E. (State of California)
  State of Florida

Learning Objectives

Name the 4 components that can be made from a unit of whole blood and describe the function of each.

Define the following terms: antigen, clinically significant antibody, hemolysis, and the complement system.

List compatible components for the following patient blood groups: A,B, O, and AB.

Define the antibody screen and explain why it is important in pre-transfusion testing.

Name 3 crossmatch tests and define when an antiglobulin crossmatch must be done.

Describe the laboratory's role in the evaluation of a suspected transfusion reaction.

Define the elements of accurate and thorough testing prior to infusion of blood components to a patient


Course Outline 
Introduction
Learning Objectives
Overview
Blood Components
Overview
Red Cells
Platelets and Plasma
Cryoprecipitate
Blood Groups
Overview
ABO and Compatibility
Rh
Pre-Transfusion Testing
Overview
Agglutination
Determining ABO/Rh Group
Other Required Pretransfusion Testing
Other Optional Pretransfusion Testing
Patterns
Anti-D
Warm Autoantibody
Antibody to High Frequency Antigen
HTLA
Anti-M
Basic Antibody ID
Overview
Exclusion/ Inclusion Criteria
Examples
Transfusion Reactions
Overview
Hemolytic Transfusion Reaction
Dangerous Reactions without Hemolysis