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Cerebrospinal Fluid

Training Course Specifics
Summary: Overview of the lumbar puncture procedure. Gross and microscopic examination of cerebrospinal fluid

Intended Audience: All laboratory staff and students

Length of Course: 1 hour

Number of Exam Questions: 10

Authors: Brent Wood, MD, PhD
Jana Roney, BS, MT(ASCP)
Adam R. Orkand, BA
Michael L. Astion, MD, PhD

Continuing Education Credit
Number of credit hours: 1 hour
Accredited by: P.A.C.E. (State of California)
  State of Florida

Learning Objectives

Describe the production and collection of cerebrospinal fluid (CSF).

Describe the gross appearance of CSF specimens.

Describe the principles of manual chamber counts, cytocentrifuge preparation, and Wright staining.

Identify the major types of normal and abnormal cells in CSF and describe their signficance.


Course Outline 
Overview
Learning Objectives
Anatomy
Specimen Collection
Laboratory Findings
Microbiology
Gross Examination
Overview
Clarity
Traumatic Tap
Procedures
Cell Count
Cytocentrifuge
Wright Stain
Gram Stain
Electrophoresis
Latex Agglutination
Microscopic Examination
Cell Types
Normal
Inflammation
Hemorrhage
Infection
Malignancy
Exams
Exam 1
Exam 2