ANCA
Instructor:
Kathleen W. Hutchinson, MS, MT(ASCP); Mark H. Wener, MD; Bruce G. Gilliland, MD; Michael L. Astion, MD, PhD
Contact Hours:
2.0
Date:
January 01, 2024 - December 31, 2025
Description:
Testing for anti-neutrophil cytoplasmic antibodies.
Learning Objectives:
- Identify the steps of an indirect immunofluorescence antibody (IFA) procedure and be able to state the purpose of each step.
- Identify the steps of an enzyme immunoassay (EIA) procedure and be able to state the purpose of each step.
- State the role of ANCA testing in the diagnosis of vasculitis.
- Identify neutrophils and lymphocytes on IFA substrates.
- View ANCA IFA results and identify C-ANCA, P-ANCA, ANA, mixed ANCA and ANA, and atypical staining patterns on ethanol fixed substrate.
- View ANCA IFA results and identify ANCA staining on formalin fixed substrate.
- State two strategies that can be used to differentiate P-ANCA, ANA staining, and mixed patterns.
- Identify the antibodies that are commonly associated with the C-ANCA and P-ANCA patterns.
- Identify the clinical correlations, if any, that are associated with ANCA staining patterns.