Laboratory Methods
Instructor:
Kathleen W. Hutchinson, MS, MT(ASCP); Mark H. Wener, MD; Bruce G. Gilliland, MD; Michael L. Astion, MD, PhD
Contact Hours:
1.0
Date:
January 01, 2024 - December 31, 2025
Description:
Microscopy, immunofluorescence assay (IFA) and enzyme immunoassay (EIA).
Learning Objectives:
- State the role of ANA testing in the diagnosis of autoimmune rheumatic diseases and list three methods used.
- Identify the steps of an indirect immunofluorescence antibody (IFA) procedure and state the purpose of each step.
- Identify the steps of an enzyme immunoassay (EIA) procedure and state the purpose of each step.
- Describe the function of various components of an epifluorescence microscope and how they influence IFA staining.